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Fasteris Life
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: MiR-30a-5p Inhibits Epithelial-to-Mesenchymal Transition and Upregulates Expression of Tight Junction Protein Claudin-5 in Human Upper Tract Urothelial Carcinoma Cells
doi: 10.3390/ijms18081826
Figure Lengend Snippet: Expression profiles of miRNAs in human UTUC tumor and non-tumor samples. Three tumor (T1–T3; light blue to navy blue) and three adjacent normal tissues (N1–N3; orange-yellow-green) were subjected to RNA extraction and NGS-miRNA sequencing through the Illumina MiSeq platform. ( A ) Heat-map clustering analysis was conducted to examine the overall miRNA expression profiles among samples. A line scatterplot was used to present miRNA expression profiles; ( B ) Downregulation of miR-30a-5p in UTUC tumor tissues ( n = 22) compared with adjacent normal controls ( n = 14) by RT-qPCR analysis. The asterisk denotes p < 0.001 using an unpaired t -test.
Article Snippet: The small RNA library construction and
Techniques: Expressing, RNA Extraction, Sequencing, Quantitative RT-PCR
Journal: Bioactive Materials
Article Title: Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis: Toward a precision therapy
doi: 10.1016/j.bioactmat.2023.06.012
Figure Lengend Snippet: miR-223 mediates the inhibitory effect of hUC-EVs on NLRP3 (A) Volcano plot illustrating miR expression of OA rat knee joint cartilage treated with or without hUC-EVs. Red dots, up-regulated miRs, blue dots, down-regulated miRs, gray dots, miRs with no significant difference. (B) Heat map of miRNA sequencing (miRNA-seq) analysis. Red arrow: miR-223. (Red: high expression, blue: low expression, n = 3 per group) (C) qRT-PCR analysis of miR-223 (n = 3 per group). (D) Binding seq of NLRP3 and miR-223. (E) Dual-luciferase reporter gene assay validating the interaction between NLRP3 and miR-223. (F) Protein levels of NLRP3 examined by western blotting. **P < 0.01. These experiments were repeated three times independently, and representative results are shown.
Article Snippet: To further investigate the underlying mechanism of hUC-EVs promoting chondrocyte survival and cartilage repair, we performed
Techniques: Expressing, Sequencing, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Gene Assay, Western Blot